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Figure 8 | Journal of Molecular Signaling

Figure 8

From: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling

Figure 8

Expression kinetics of CLU mRNA and protein levels after induction of dnTCF1 in LS174T cells. Expression kinetics of CLU mRNA and protein levels were studied 0, 3, 6, and 12 hr after induction of dnTCF1 in LS714T cells. c-MYC and p21CIP1/WAF1 mRNA levels were also monitored. (A) Western blot with cell lysates from LS174T cells 0, 3, 6, and 12 hr after induction. CLU protein levels do not increase until 12 hr after induction. Exogenous dnTCF1 proteins are induced as early as 3 hr after induction, and c-MYC protein levels decrease in parallel. (B) Real time RT-PCR shows that CLU34 and p21CIP1/WAF1 mRNA levels do not increase until 12 hr after induction. c-MYC mRNA levels decrease 6 hr after induction. Data are presented as the mean ± standard deviation from 2 separate experiments with each experiment consisting of the mean value of 3 independent determinations.

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