Figure 7From: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling Alterations of CLU, p21, and c-MYC mRNA levels after induction of dnTCFs in LS174T cells. The expression levels of various transcripts were monitored by real time RT-PCR after induction of dnTCFs in LS174T derived cell lines. Expression levels were normalized to the Ubiquitin C (UBC) transcript. The CLU34 mRNA variant is specifically up-regulated in response to dnTCF1 over-expression in LS174T cells. Data are presented as the mean ± standard deviation from 2 separate experiments with each experiment consisting of the mean value of 3 independent determinations. c-MYC mRNA levels decrease, whereas p21CIP1/WAF1 mRNA levels increase after induction of both dnTCFs.Back to article page