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Figure 6 | Journal of Molecular Signaling

Figure 6

From: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling

Figure 6

Characterization of the 5'-UTR of the CLU34 transcript by 5'-end RACE and RT-PCR. A colon adenocarcinoma cDNA library was used for 5'-end rapid amplification of cDNA ends (RACE) to determine the sequence of the extreme 5'-end of CLU34 mRNA. None of the three cloned 5'-end RACE fragments or the RT PCR analysis supported the most 5' extreme end of the CLU34 transcript containing the putative TATA-box as suggested by the RefSeq database. The 5'-end RACE results were consistent with results from RT-PCR reactions which only yielded an amplification product if the sense primer was placed downstream of the putative TATA-box.

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