Figure 4

Cell-based TGFβ activity assay. Cells in which RGMa, RGMb, or RGMc levels were knocked down were treated with TGFβ and assayed for CAGA-luc activity. Knockdown of TGFβ RII was also performed as a positive control. Error bars represent the standard deviation (n = 4). The TGFβ-induced response from the RGM and scramble siRNA-treated cells showed no significant difference from each other, while the TGFβ R2 siRNA-treated cells showed significantly lower responsiveness, as determined by a two-tailed Student's T-test (p value threshold of 0.05).