Figure 2

Cell-based BMP activity assays. Cells in which RGMa (A), RGMb (B), or RGMc (C) levels were knocked down were treated with either BMP2 or BMP12 and assayed for ALP activity or BRE-luc activity. In each case, data from two effective siRNA duplexes are shown compared to the scramble siRNA sequence. In all cases, activities resulting from BMP2 or BMP12 treatment are normalized to the activity of untreated cells. Error bars represent the standard deviation (n = 4). In all cases, the BMP-induced response from the RGM siRNA-treated cells was significantly lower than for the scramble siRNA-treated cells, as determined by a two-tailed Student's T-test (p-value threshold of 0.05).