Journal of Molecular Signaling

Table 3 Effect of RGS4 on E2F8 signaling

From: E2F8 is a nonreceptor activator of heterotrimeric G proteins

ADE2 plasmid	URA3 plasmid	Signaling	Fluorescence
C5aR NQ	Vector	++++	-
	RGS4-GFP	+	+
E2F8	Vector	+++++++	-
	RGS4-GFP	+	+
Vector	Vector	-	-
	RGS4-GFP	-	+

Yeast BY1142 was transformed with the indicated plasmids. Three transformants per condition were replicated onto selective plates containing 3-AT and assessed for growth after 3 days. The strength of signaling and fluorescence is reported as in Table 1.

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