E2F8 effect on receptor-mediated signaling. Yeast BY1142 was transformed with a PFUS1-LacZ reporter construct (TRP1 selection), then transformed with plasmids encoding E2F8 or empty vector (ADE2 selection) and C5aR or empty vector (URA3 selection). The agonist W5Cha was used to activate the receptor. After four hours of ligand treatment, cells were lysed and β-galactosidase activity was measured by colorimetry. This experiment is representative of three independent trials, each of which examined three transformants per condition.