Figure 8
From: Agonist mediated internalization of M2 mAChR is β-arrestin-dependent
Internalized M 2 mAChRs exhibit a differential affinity for β-arrestin 2-GFP compared to other muscarinic subtypes. HeLa cells were transiently co-transfected with plasmids encoding β-arrestin 2-GFP and either HA-tagged M1, M3, M4, M5 mAChR or FLAG-tagged M2 mAChR. Cells were untreated or treated with 1 mM carbachol for 0 min or 30 min. Grayscale image indicates β-arrestin 2-GFP localization while the upper right inset indicates immunostaining of the mAChR in a small section of the cell (outline). Arrows indicate overlap between internalized M2 mAChRs and β-arrestin 2-GFP. Overlay represents co-immunostaining of mAChR (red) and β-arrestin 2-GFP expression (green) and their colocalization (yellow).