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Figure 5 | Journal of Molecular Signaling

Figure 5

From: Agonist mediated internalization of M2 mAChR is β-arrestin-dependent

Figure 5

Agonist-promoted internalized FLAG-tagged M 2 mAChRs exhibit overlap with β-arrestin 2-GFP at intracellular sites in various cell lines. Cells were transiently transfected with FLAG-tagged M2 mAChR and β-arrestin 2-GFP and treated with 1 mM carbachol for 30 min at 37°C. Co-localization of β-arrestin 2-GFP with internalized M2 mAChRs occurred in HeLa, COS-7 and rat aortic smooth muscle cells (RASMCs). Arrows indicate overlap between β-arrestin 2-GFP and M2 mAChRs in intracellular compartments. Confocal images are representative of three independent experiments.

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