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Figure 4 | Journal of Molecular Signaling

Figure 4

From: Agonist mediated internalization of M2 mAChR is β-arrestin-dependent

Figure 4

Stimulation of M 2 mAChRs leads to stable co-localization of β-arrestin 2-GFP at intracellular sites. MEF wild type or KO1/2 cells were transiently co-transfected with the human FLAG-tagged M2 mAChR and β-arrestin 2-GFP constructs. Following 30 minutes of 1 mM carbachol stimulation, cells were fixed and processed for indirect immunofluorescence as described in the Methods. Localization of β-arrestin 2-GFP and M2 mAChR was visualized by confocal microscopy. Confocal images are representative of three independent experiments.

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