DA uptake is inhibited by 10 nM E
in PC12 cells. Estradiol (E2), vs. ethanol vehicle (Control) was added to the cells together with 3H-DA for a 30 minute incubation, * = significantly different from vehicle control at p < 0.05. A. Cells were serum-starved for 48 hours with no NGFβ treatment. Total cellular uptake of DA over a 30 min period ± E2 treatment was monitored. B. Cells were serum-starved while being treated for 2 days ± 20 ng/ml NGFβ. DAT-specific DA uptake was measured over a 30 min period ± E2 treatment. NET- and DAT-blocking drugs were included to evaluate DAT-specific uptake of DA. # = significantly different vs. NGFβ-treated control (p < 0.05). C. The rapid effects of E2 treatment on 3H-DA uptake were shown when E2 treatment was added only during the last 5 minutes of the 30 min uptake assay. # = significantly different vs. NGFβ-treated vehicle control (p < 0.05).