Skip to main content
Figure 6 | Journal of Molecular Signaling

Figure 6

From: Tyrosine-specific MAPK phosphatases and the control of ERK signaling in PC12 cells

Figure 6

Wild type and non-phosphorylatable PTPPBSγ affect ERK activity. A) Expression analysis of wild type and mutant PTPPBSγ proteins. Equal amounts of protein from PC12 cells stably expressing wild type (WT) or mutant (ΔKIM, D309A, S94A/T114A, and C343S) PTPPBSγ were subjected to SDS-PAGE and immunoblot analysis using antibody 6A6. B) EGF-induced ERK1/2 activity in PC12 cells stably expressing PTPPBSγ protein variants. Serum starved cells that stably express PTPPBSγ mutants were stimulated with 10 ng/ml EGF for 2 or 5 min, and lysed. Relative phospho-ERK1/2 levels were determined via immunoblot analysis as described in the legends to Fig. 2. Results are presented as mean values ± SEM (n = 4, Student's t-test; *p < 0.05).

Back to article page