Skip to main content
Figure 3 | Journal of Molecular Signaling

Figure 3

From: Tyrosine-specific MAPK phosphatases and the control of ERK signaling in PC12 cells

Figure 3

Expression of PTPRR isoforms in PC12 cells. A) PC12 cells were retrovirally transduced with expression constructs for mouse PTPRR isoforms, or mock-transduced. Equal amounts of cell lysates were subjected to 10% SDS-PAGE and immunoblotting. Rat PCPTP1, mouse PTPBR7 and mouse PTPPBSγ (indicated on the left) were detected using 6A6 antibody. Molecular size markers are indicated on the right. The arrow indicates a cross-reactive ~50 kDa background band. B) Quantitative representation of PTPRR expression levels as determined in A. PTPRR levels were corrected for loading differences using monoclonal α-tubulin antibody E7 immunostaining (not shown) as a control. Results are the mean values ± SEM of four individual pools of cells. C) Subcellular localization of stably expressed PTPRR protein isoforms in the transduced PC12 cells as determined by fluorescence microscopy using polyclonal α-SL antibody. Bar indicates 10 μm.

Back to article page