Figure 3From: Tyrosine-specific MAPK phosphatases and the control of ERK signaling in PC12 cells Expression of PTPRR isoforms in PC12 cells. A) PC12 cells were retrovirally transduced with expression constructs for mouse PTPRR isoforms, or mock-transduced. Equal amounts of cell lysates were subjected to 10% SDS-PAGE and immunoblotting. Rat PCPTP1, mouse PTPBR7 and mouse PTPPBSγ (indicated on the left) were detected using 6A6 antibody. Molecular size markers are indicated on the right. The arrow indicates a cross-reactive ~50 kDa background band. B) Quantitative representation of PTPRR expression levels as determined in A. PTPRR levels were corrected for loading differences using monoclonal α-tubulin antibody E7 immunostaining (not shown) as a control. Results are the mean values ± SEM of four individual pools of cells. C) Subcellular localization of stably expressed PTPRR protein isoforms in the transduced PC12 cells as determined by fluorescence microscopy using polyclonal α-SL antibody. Bar indicates 10 μm.Back to article page