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Figure 3 | Journal of Molecular Signaling

Figure 3

From: Differential partitioning of Gαi1 with the cellular microtubules: a possible mechanism of development of Taxol resistance in human ovarian carcinoma cells

Figure 3

PKA activity in the parental and taxol resistant cells before and after treatment with taxol. PKA activity was determined using the Pierce Colorimetric Assay Kit that utilizes a fluorescent-labeled Kempeptide (a PKA-specific peptide (LRRASLG) substrate). Untreated and taxol treated cells were homogenized in a Dounce homogenizer (10 strokes with a tight fitting pestle) in a buffer containing 25 mM Tris-HCl, pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 10 mM DTT and protease inhibitor cocktail. The homogenate was centrifuged at 13,000 × g for 20 min and the PKA activity was measured (in triplicate) in the supernatant fraction. Values shown are mean ± SD of 3 separate experiments.

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